Useful Links & Tools
- MSDS
- Specification Sheet
- Certificate of Analysis
- Certificate of Origin
- C3710 - Datasheet (22 KB)
- JAKs (166 KB )
- Cytokine Receptors Hematopoetin Receptor Family (259 KB )
- Bulk Quote
C3710
Sigma
Ciliary Neurotrophic Factor human
recombinant, expressed in Escherichia coli, lyophilized powder, cell culture tested
| Synonym: | CNTF |
| MDL number: | MFCD00676720 |
Description
| Analysis Note | The proliferative activity is tested in a cell proliferation assay using the cytokine-dependent human erythroleukemic cell line, TF-1. |
| Physical form | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin. |
| Biochem/physiol Actions | Ciliary neurotrophic factor was first identified as a survival factor for neurons from the ciliary ganglion of chicken embryos. Most of its known actions are restricted to cells of the nervous system, including motor neurons, sympathetic ganglion neurons, sensory neurons, hippocampal neurons, and medial septal neurons. CNTF also prevents degeneration of motor axons after axotomy and promotes astrocyte differentiation and oligodendrocyte survival and maturation. Outside the nervous system, CNTF maintains embryonic stem cells in an undifferentiated, pluripotent state. CNTF is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). CNTF exerts its actions through the activation of the high-affinity CTNF receptor complex, which contains the ligand-binding α-subunit (CNTF Rα) and two signal transducing β-subunits (LIF Rβ and gp130). The LIF Rβ subunit is also shared by receptors for LIF and OSM. The gp130 subunit is also shared by receptors for LIF, OSM, IL-6, and IL-11. CNTF is localized in the cell nucleus subsequent to receptor binding. Human and rat CNTF share ~83% sequence homology and show cross-reactivity in bioactivity. |
Properties
| recombinant | expressed in Escherichia coli |
| assay | ≥97% (SDS-PAGE) |
| form | lyophilized powder |
| potency | 20-250 ng/mL ED50/EC50 |
| mol wt | mol wt 22.8 kDa |
| total impurities | Endotoxin, tested |
| suitability | cell culture tested |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| reference | Callard, R., and Gearing, A. The Cytokine Facts Book New York , (1994) |
| Yancopoulos, G.D., Nicola, N., ed., Ciliary neurotrophic factor. Guidebook to Cytokines and Their Receptors New York , (1994), 134-137 | |
| Yancopoulos, G.D., Nicola, N., ed., The ciliary neurotrophic factor (CNTF) receptor complex. Guidebook to Cytokines and Their Receptors New York , (1994), 134-137 | |
| Heim, M.H., The Jak-STAT pathway: cytokine signalling from the receptor to the nucleus. Receptor Signal Transduction Res. 19, 75-120, (1999) | |
| Mertani, H.C., et al., Cytoplasmic and nuclear cytokine receptor complexes. Vitam. Horm. 57, 79-121, (1999) | |
| Kitamura, T., Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3 or erythropoietin. J. Cell Physiol. 140, 323, (1989) |
